Honors and Awards
Area of Specialization
Dr. Prashant Kumar received his Ph.D. in 2009 from the Max Planck Institute for Infection Biology, Department of Molecular Biology, Berlin, Germany. Following this, Dr. Kumar received postdoctoral training in the laboratory of Prof. Karl Simin at the Department of Cancer Biology, University of Massachusetts (UMMS) Medical School, Worcester, USA, where he established a novel mouse model of malignant breast cancers that required improved therapeutic strategies, and was involved in studying the clinical impact of breast cancer. In his work, he showed that mammary tumors caused by the inactivation of the pRb family (pRbf) of proteins (pRb, p107, p130), together with Brca1 and p53 inactivation, mimicked several aspects of the most aggressive forms of breast cancer. This finding illustrated the compounding effect of acquiring multiple tumor suppressor mutations during tumor evolution and underscores the distinct requirements of each of these canonical tumor suppressor proteins. In June 2012, he moved to Singapore, continuing his research at the Institute of Molecular and Cell Biology (IMCB), A-STAR, in the laboratory of Prof. Jean Paul Thiery, a world renowned authority in the area of EMT (Epithelial-Mesenchymal Transition). During this period, the focus of his work took on a more translational aspect, and he was involved in the development of a novel point-of-care (POC) diagnostic platform for the detection of multiple protein bladder cancer biomarkers. He was also involved in the establishment of a culture method to assess breast cancer circulating tumor cells (CTCs) harvested from blood samples of patients undergoing neoadjuvant therapy. This new technique provides an opportunity to analyze CTC clonal heterogeneity and adapt therapeutic modalities in refractory breast cancer patients. At IOB, his work is now centred on biomarker discovery for bladder cancers, using state-of-the-art quantitative proteomics approaches. He is also interested in defining the molecular pathways that underlie the malignancy associated with bladder carcinoma, with an emphasis on the biological mechanisms and regulatory relationship of EMT in cancer progression.
Major Research Areas
In vivo mouse model and clinical impact associated with bladder/breast/head and neck/gastric cancer(s)
Research papers: 12; Book chapters: 1;
Leonard, J. L., Leonard, D. M., Wolfe, S. A., Liu, J.,
Rivera, J., Yang, M., Leonard, R. T., Johnson, J. P. S., Kumar, P.,
Liebmann, K. L., Tutto, A. A., Mou, Z., Simin, K. J. (2017).
The Dkk3 gene encodes a vital intracellular regulator of cell proliferation. PLoS One. 12, e0181724. [PubMed]
Yadavalli, S., Jayaram, S., Manda, S.
S., Madugundu, A. K., Nayakanti, D. S., Tan, T. Z., Bhat, R., Rangarajan,
A., Chatterjee, A., Gowda, H., Thiery, J. P. and Kumar, P.* (2017). Data-Driven Discovery of Extravasation Pathway in Circulating Tumor
7, 43710. [PubMed] (*Corresponding Author)
Radhakrishnan, A., Nanjappa, V., Raja, R., Sathe, G. J., Chavan, S., Nirujogi, R. S., Patil, A., Solanki, H., Renuse, S., Sahasrabuddhe, N. A., Mathur, P. P., Prasad, T. S. K., Kumar, P., Califano, J. A., Sidransky, D., Pandey, A., Gowda, H. and Chatterjee, A. (2016). Dysregulation of splicing proteins in head and neck squamous cell carcinoma. Cancer Biology and Therapy. 17, 219-29. [PubMed]
Wu, F†., Kumar, P†., Lu, C., El Marjou, A., Qiu, W., Lim, C. T., Thiery, J. P. and Liu, R. (2015). Homophilic Interaction and Deformation of E-cadherin and Cadherin 7 Probed by Single Molecule Force Spectroscopy. Archives of Biochemistry and Biophysics. 587, 38-47. [PubMed] †These authors contributed equally to this project
Khoo, B. L†., Lee, C. S†., Kumar, P†., Tan, Z. T., Warkiani, E. M., Ow, GW. S., Nandi, S., Lim, T. C. and Thiery, P. J. (2015). Short-term expansion of breast circulating cancer cells predicts response to anti-cancer therapy. Oncotarget. [PubMed] †These authors contributed equally to this project
Kumar, P., Nandi. S., Tan, Z. T., Ler, G. S., Seng, K. C., Yen, W. L., Vordos, D., laTaille, D. A., Raida, M., Beyer, B., Ricci, E., Colombel, M., Chong, W. T., Chiong, E., Soo, R., Park, K. M., Ha, K. H., Gunaratne, J. and Thiery, P. J. (2015). Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder. Oncotarget. 6:13539-49. [PubMed]
Akalay, I., Tan, T. Z., Kumar, P., Janji, B., Mami-Chouaib, F., Charpy, C., Vielh, P., Larsen, A. K., Thiery, J. P., Sabbah, M. and Chouaib, S. (2015). Targeting WNT1-inducible signaling pathway protein 2 alters human breast cancer cell susceptibility to specific lysis through regulation of KLF-4 and miR-7 expression. Oncogene. 34:2261-2271. [PubMed]
Johnson, J. P., Kumar, P., Koulnis, M., Patel, M. and Simin, K. (2014). Crucial and Novel Cancer Drivers in a Mouse Model of Triple-Negative Breast Cancer. Cancer Genomics Proteomics. 11:115-126. [PubMed]
Goel, L. H., Pursell, B., Chang, C., Shaw, L., Mao, J., Simin, K., Kumar, P., Kooi, C., Shultz, L., Greiner, D., Norum, J., Toftgard, R., Kuperwasser, C. and Mercurio, M. A. (2013). GLI1 regulates a novel neuropilin-2/a6ß1 integrin based autocrine pathway that contributes to breast cancer initiation. EMBO Mol Med 5:488-508. [PubMed]
Kumar, P., Mukherjee, M., Johnson, J. P., Patel, M., Huey, B., Albertson, D. G. and Simin, K. (2012). Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8. [PubMed]
Singh, D. D., Saikrishnan, K., Kumar, P., Surolia, A., Sekar, K. and Vijayan, M. (2005). Glycobiology. Unusual sugar specificity of banana lectin from Musa paradisiaca and its probable evolutionary origin. 15:1025-1032. [PubMed]
Singh, D. D., Saikrishnan, K., Kumar, P., Dauter, Z., Sekar, K., Surolia, A. and Vijayan, M. (2004). Purification, crystallization and preliminary X-ray structure analysis of the banana lectin from Musa paradisiaca. Acta Crystallogr D Biol Crystallogr 60:2104-2106. [PubMed]