| Comprehensive Gene Expression Profiling of Meningitis using Whole Human Genome Array
The prevalence of tuberculous meningitis in developing countries such as India remains high, primarily because of the rise in HIV infection. Scientists at IOB are involved in the identification of molecular signatures using genome microarrays of pathogen and host interaction in tuberculous meningitis. These molecules will provide a better understanding host-pathogen interaction, diagnosis, disease pathogenesis and novel targets for therapeutic intervention in different type of meningitis.
Tuberculous meningitis (TBM) is an infection of the meninges (membranes covering the brain and spinal cord) caused by the bacteria Mycobacterium tuberculosis that causes tuberculosis. In developing countries like India, TBM is still a major cause of neurological disabilities and death. TBM is a serious, often fatal disease. Early Diagnosis of TBM can often be a challenge, time consuming, costly, and some times problematic also. Early detection of TBM offers many therapeutic options, but there are no reliable markers that are currently available. Gene expressional analysis of complex biological samples using Microarrays become feasible in recent years. In this study, our objective was to discover potential biomarkers for TBM by using the MicroArrays and subsequently validate them using Quantitative Real-Time- PCR and Immunohistochemical labeling of tissue microarrays.
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